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Insights into SusCD-mediated glycan import by a prominent gut symbiont

Gray, Declan A. and White, Joshua B. R. and Oluwole, Abraham O. and Rath, Parthasarathi and Glenwright, Amy J. and Mazur, Adam and Zahn, Michael and Baslé, Arnaud and Morland, Carl and Evans, Sasha L. and Cartmell, Alan and Robinson, Carol V. and Hiller, Sebastian and Ranson, Neil A. and Bolam, David N. and van den Berg, Bert. (2021) Insights into SusCD-mediated glycan import by a prominent gut symbiont. Nature Communications, 12 (1). p. 44.

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Official URL: https://edoc.unibas.ch/80736/

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Abstract

In Bacteroidetes, one of the dominant phyla of the mammalian gut, active uptake of large nutrients across the outer membrane is mediated by SusCD protein complexes via a "pedal bin" transport mechanism. However, many features of SusCD function in glycan uptake remain unclear, including ligand binding, the role of the SusD lid and the size limit for substrate transport. Here we characterise the β2,6 fructo-oligosaccharide (FOS) importing SusCD from Bacteroides thetaiotaomicron (Bt1762-Bt1763) to shed light on SusCD function. Co-crystal structures reveal residues involved in glycan recognition and suggest that the large binding cavity can accommodate several substrate molecules, each up to ~2.5 kDa in size, a finding supported by native mass spectrometry and isothermal titration calorimetry. Mutational studies in vivo provide functional insights into the key structural features of the SusCD apparatus and cryo-EM of the intact dimeric SusCD complex reveals several distinct states of the transporter, directly visualising the dynamics of the pedal bin transport mechanism.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Hiller)
UniBasel Contributors:Hiller, Sebastian
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
e-ISSN:2041-1723
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:31 Aug 2021 13:57
Deposited On:31 Aug 2021 13:57

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