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Cost-effective capillary electrophoresis with contactless conductivity detection for quality control of beta-lactam antibiotics

Nguyen, Thi Anh Huong and Pham, Thi Ngoc Mai and Le, Thai Binh and Le, Dinh Chi and Tran, Thi Thanh Phuong and Nguyen, Thi Quynh Hoa and Nguyen, Thi Kim Thuong and Hauser, Peter C. and Mai, Thanh Duc. (2019) Cost-effective capillary electrophoresis with contactless conductivity detection for quality control of beta-lactam antibiotics. Journal of Chromatography A, 1605. p. 360356.

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Official URL: https://edoc.unibas.ch/80537/

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Abstract

A simple and inexpensive approach for determination of various antimicrobial drugs using a purpose-made compact capillary electrophoresis (CE) instrument with capacitively coupled contactless conductivity detection ((CD)-D-4) is reported. The objective of the work is to propose an affordable and easily-implemented tool for quality control and detection of counterfeiting of antibiotic formulations in resource constrained developing countries. The design of the purpose-made CE-(CD)-D-4 system was improved according to the feedback from over 10 years of use of our previous instrument. CE-(CD)-D-4 methods were for the first time developed to analyze beta-lactam-based antibiotics commonly used in Vietnam, including single- beta-lactam antibiotics (i.e. Cephalexin, Cefotaxime Sodium, Cefixime and Sulbactam) as well as beta-lactams co-formulated with Sulbactam (i.e. Amoxicillin, Ampicillin, Cefoperazone and Sulbactam). Single beta-lactam antibiotics were analyzed using a background electrolyte (BGE) composed of Tris/Ace (10 mM, pH 7.8) whereas beta-lactam- Sulbactam combinations were simultaneously separated using a BGE containing Tris/Ace (10 mM, pH 7.5). The best achieved detection limits were 2.0 mg/L and 1.0 mg/L for these two groups, respectively. Good agreement between results obtained from CE-(CD)-D-4 and standard confirmation methods (LC MS) was achieved, with a coefficient of determination, r(2), of 0.9991. The applicability of the developed CE-(CD)-D-4 method was demonstrated for quality control of 24 beta-lactam-based antimicrobial drugs available in Vietnam. (C) 2019 Elsevier B.V. All rights reserved.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser)
UniBasel Contributors:Hauser, Peter C. P. C.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:0021-9673
e-ISSN:1873-3778
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:16 Feb 2021 11:49
Deposited On:16 Feb 2021 11:45

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