The tight junctions protein Claudin-5 limits endothelial cell motility

Yang, Zhenguo and Wu, Shuilong and Fontana, Federica and Li, Yanyu and Xiao, Wei and Gao, Zhangdai and Krudewig, Alice and Affolter, Markus and Belting, Heinz-Georg and Abdelilah-Seyfried, Salim and Zhang, Jingjing. (2021) The tight junctions protein Claudin-5 limits endothelial cell motility. Journal of Cell Science, 134 (1). jcs248237.

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Official URL: https://edoc.unibas.ch/79996/

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Steinberg's differential adhesion hypothesis suggests that adhesive mechanisms are important for sorting of cells and tissues during morphogenesis (Steinberg, 2007). During zebrafish vasculogenesis, endothelial cells sort into arterial and venous vessel beds but it is unknown whether this involves adhesive mechanisms. Claudins are tight junction proteins regulating the permeability of epithelial and endothelial tissue barriers. Previously, the roles of Claudins during organ development have exclusively been related to their canonical functions in determining paracellular permeability. Here, we use atomic force microscopy to quantify Claudin-5-dependent adhesion and find that this strongly contributes to the adhesive forces between arterial endothelial cells. Based on genetic manipulations, we reveal a non-canonical role of Claudin-5a during zebrafish vasculogenesis, which involves the regulation of adhesive forces between adjacent dorsal aortic endothelial cells.; In vitro; and; in vivo; studies demonstrate that loss of Claudin-5 results in increased motility of dorsal aorta endothelial cells and in a failure of the dorsal aorta to lumenize. Our findings uncover a novel role of Claudin-5 in limiting arterial endothelial cell motility, which goes beyond its traditional sealing function during embryonic development.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell Biology (Affolter)
UniBasel Contributors:Affolter, Markus
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Company of Biologists
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:17 Nov 2021 08:05
Deposited On:17 Nov 2021 08:05

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