edoc

Dynamics in protein translation sustaining T cell preparedness

Wolf, Tobias and Jin, Wenjie and Zoppi, Giada and Vogel, Ian A. and Akhmedov, Murodzhon and Bleck, Christopher K. E. and Beltraminelli, Tim and Rieckmann, Jan C. and Ramirez, Neftali J. and Benevento, Marco and Notarbartolo, Samuele and Bumann, Dirk and Meissner, Felix and Grimbacher, Bodo and Mann, Matthias and Lanzavecchia, Antonio and Sallusto, Federica and Kwee, Ivo and Geiger, Roger. (2020) Dynamics in protein translation sustaining T cell preparedness. Nature immunology, 21 (8). pp. 927-937.

[img] PDF
Restricted to Repository staff only

14Mb

Official URL: https://edoc.unibas.ch/79811/

Downloads: Statistics Overview

Abstract

In response to pathogenic threats, naive T cells rapidly transition from a quiescent to an activated state, yet the underlying mechanisms are incompletely understood. Using a pulsed SILAC approach, we investigated the dynamics of mRNA translation kinetics and protein turnover in human naive and activated T cells. Our datasets uncovered that transcription factors maintaining T cell quiescence had constitutively high turnover, which facilitated their depletion following activation. Furthermore, naive T cells maintained a surprisingly large number of idling ribosomes as well as 242 repressed mRNA species and a reservoir of glycolytic enzymes. These components were rapidly engaged following stimulation, promoting an immediate translational and glycolytic switch to ramp up the T cell activation program. Our data elucidate new insights into how T cells maintain a prepared state to mount a rapid immune response, and provide a resource of protein turnover, absolute translation kinetics and protein synthesis rates in T cells ( https://www.immunomics.ch ).
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Bumann)
UniBasel Contributors:Bumann, Dirk
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Research
ISSN:1529-2908
e-ISSN:1529-2916
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
edoc DOI:
Last Modified:30 Dec 2020 13:59
Deposited On:30 Dec 2020 13:27

Repository Staff Only: item control page