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Monitoring microbial population dynamics at low densities

Julou, Thomas and Desprat, Nicolas and Bensimon, David and Croquette, Vincent. (2012) Monitoring microbial population dynamics at low densities. Review of Scientific Instruments, 83 (7). 074301.

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Official URL: https://edoc.unibas.ch/79789/

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Abstract

We propose a new and simple method for the measurement of microbial concentrations in highly diluted cultures. This method is based on an analysis of the intensity fluctuations of light scattered by microbial cells under laser illumination. Two possible measurement strategies are identified and compared using simulations and measurements of the concentration of gold nanoparticles. Based on this comparison, we show that the concentration of Escherichia coli and Saccharomyces cerevisiae cultures can be easily measured in situ across a concentration range that spans five orders of magnitude. The lowest measurable concentration is three orders of magnitude (1000×) smaller than in current optical density measurements. We show further that this method can also be used to measure the concentration of fluorescent microbial cells. In practice, this new method is well suited to monitor the dynamics of population growth at early colonization of a liquid culture medium. The dynamic data thus obtained are particularly relevant for microbial ecology studies.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Bioinformatics (van Nimwegen)
UniBasel Contributors:Julou, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:AIP Publishing
ISSN:0034-6748
e-ISSN:1089-7623
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:16 Dec 2020 15:02
Deposited On:16 Dec 2020 15:02

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