Merz, Katharina and Hondele, Maria and Goetze, Hannah and Gmelch, Katharina and Stoeckl, Ulrike and Griesenbeck, Joachim. (2008) Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules. Genes & development, 22 (9). pp. 1190-1204.
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Abstract
Synthesis of ribosomal RNAs (rRNAs) is the major transcriptional event in proliferating cells. In eukaryotes, ribosomal DNA (rDNA) is transcribed by RNA polymerase I from a multicopy locus coexisting in at least two different chromatin states. This heterogeneity of rDNA chromatin has been an obstacle to defining its molecular composition. We developed an approach to analyze differential protein association with each of the two rDNA chromatin states in vivo in the yeast Saccharomyces cerevisiae. We demonstrate that actively transcribed rRNA genes are largely devoid of histone molecules, but instead associate with the high-mobility group protein Hmo1.
Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Hondele) |
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UniBasel Contributors: | Hondele, Maria |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Cold Spring Harbor Laboratory Press |
ISSN: | 0890-9369 |
e-ISSN: | 1549-5477 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Language: | English |
Identification Number: |
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edoc DOI: | |
Last Modified: | 07 Dec 2020 09:15 |
Deposited On: | 07 Dec 2020 09:15 |
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