Catch me if you can: how the histone chaperone FACT capitalizes on nucleosome breathing

Hondele, Maria and Ladurner, Andreas G.. (2013) Catch me if you can: how the histone chaperone FACT capitalizes on nucleosome breathing. Nucleus, 4 (6). pp. 443-449.

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Nucleosomes confer a barrier to processes that require access to the eukaryotic genome such as transcription, DNA replication and repair. A variety of ATP-dependent nucleosome remodeling machines and ATP-independent histone chaperones facilitate nucleosome dynamics by depositing or evicting histones and unwrapping the DNA. It is clear that remodeling machines can use the energy from ATP to actively destabilize, translocate or disassemble nucleosomes. But how do ATP-independent histone chaperones, which "merely" bind histones, contribute to this process? Using our recent structural analysis of the conserved and essential eukaryotic histone chaperone FACT in complex with histones H2A-H2B as an example, we suggest that FACT capitalizes on transiently exposed surfaces of the nucleosome. By binding these surfaces, FACT stabilizes thermodynamically unfavorable intermediates of the intrinsically dynamic nucleosome particle. This makes the nucleosome permissive to DNA and RNA polymerases, providing temporary access, passage, and read-out.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Hondele)
UniBasel Contributors:Hondele, Maria
Item Type:Article
Article Subtype:Research Article
Publisher:Taylor & Francies
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:07 Dec 2020 08:26
Deposited On:07 Dec 2020 08:26

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