Multifunctional Protein Materials and Microreactors using Low Complexity Domains as Molecular Adhesives

Faltova, Lenka and Küffner, Andreas M. and Hondele, Maria and Weis, Karsten and Arosio, Paolo. (2018) Multifunctional Protein Materials and Microreactors using Low Complexity Domains as Molecular Adhesives. ACS nano, 12 (10). pp. 9991-9999.

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Recent findings indicate that a class of disordered amino acid sequences promotes functional phase transition of biomolecules in nature. Such sequences consist of low complexity domains (LCDs) that are rich in specific amino acids. In this work, we exploit these sequences by conjugating them to soluble globular domains to develop molecular adhesives that enable sensitive, controlled self-assembly of these proteins into supramolecular architectures. In particular, we used the enzyme adenylate kinase and the green fluorescent protein as soluble domains, and we show that the addition of low complexity regions induces the formation of protein particles via a multistep process. This multistep pathway involves an initial liquid-liquid phase transition, which creates protein-rich droplets that mature into protein aggregates over time. These protein aggregates consist of permeable structures that maintain activity and release active soluble proteins. We show that the LCDs dictate specific noncovalent intermolecular interactions and phase properties that are largely independent of the given globular domain. We further demonstrate that this feature, together with the dynamic state of the initial dense liquid phase, allows one to directly assemble different globular domains within the same architecture, thereby enabling the generation of both static multifunctional biomaterials and dynamic microscale bioreactors.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Hondele)
UniBasel Contributors:Hondele, Maria
Item Type:Article, refereed
Article Subtype:Research Article
Publisher: American Chemical Society
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:04 Dec 2020 17:01
Deposited On:04 Dec 2020 17:01

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