ATPase activity of the DEAD-box protein Dhh1 controls processing body formation

Mugler, Christopher Frederick and Hondele, Maria and Heinrich, Stephanie and Sachdev, Ruchika and Vallotton, Pascal and Koek, Adriana Y. and Chan, Leon Y. and Weis, Karsten. (2016) ATPase activity of the DEAD-box protein Dhh1 controls processing body formation. eLife, 5. e18746.

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Official URL: https://edoc.unibas.ch/79467/

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Translational repression and mRNA degradation are critical mechanisms of posttranscriptional gene regulation that help cells respond to internal and external cues. In response to certain stress conditions, many mRNA decay factors are enriched in processing bodies (PBs), cellular structures involved in degradation and/or storage of mRNAs. Yet, how cells regulate assembly and disassembly of PBs remains poorly understood. Here, we show that in budding yeast, mutations in the DEAD-box ATPase Dhh1 that prevent ATP hydrolysis, or that affect the interaction between Dhh1 and Not1, the central scaffold of the CCR4-NOT complex and an activator of the Dhh1 ATPase, prevent PB disassembly; in vivo; . Intriguingly, this process can be recapitulated; in vitro; , since recombinant Dhh1 and RNA, in the presence of ATP, phase-separate into liquid droplets that rapidly dissolve upon addition of Not1. Our results identify the ATPase activity of Dhh1 as a critical regulator of PB formation.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Hondele)
UniBasel Contributors:Hondele, Maria
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:eLife Sciences Publications
Note:Publication type according to Uni Basel Research Database: Journal article
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edoc DOI:
Last Modified:04 Dec 2020 16:51
Deposited On:04 Dec 2020 16:51

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