Dynamics of Synthetic Membraneless Organelles in Microfluidic Droplets

Linsenmeier, Miriam and Kopp, Marie R. G. and Grigolato, Fulvio and Emmanoulidis, Leonidas and Liu, Dany and Zürcher, Dominik and Hondele, Maria and Weis, Karsten and Capasso Palmiero, Umberto and Arosio, Paolo. (2019) Dynamics of Synthetic Membraneless Organelles in Microfluidic Droplets. Angewandte Chemie International Edition, 58 (41). pp. 14489-14494.

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Official URL: https://edoc.unibas.ch/79411/

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Cells can form membraneless organelles by liquid-liquid phase separation. As these organelles are highly dynamic, it is crucial to understand the kinetics of these phase transitions. Here, we use droplet-based microfluidics to mix reagents by chaotic advection and observe nucleation, growth, and coarsening in volumes comparable to cells (pL) and on timescales of seconds. We apply this platform to analyze the dynamics of synthetic organelles formed by the DEAD-box ATPase Dhh1 and RNA, which are associated with the formation of processing bodies in yeast. We show that the timescale of phase separation decreases linearly as the volume of the compartment increases. Moreover, the synthetic organelles coarsen into one single droplet via gravity-induced coalescence, which can be arrested by introducing a hydrogel matrix that mimics the cytoskeleton. This approach is an attractive platform to investigate the dynamics of compartmentalization in artificial cells.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Hondele)
UniBasel Contributors:Hondele, Maria
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:16 Dec 2020 15:15
Deposited On:16 Dec 2020 15:15

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