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Single particle cryo-EM of the complex between interphotoreceptor retinoid-binding protein and a monoclonal antibody

Sears, Avery E. and Albiez, Stefan and Gulati, Sahil and Wang, Benlian and Kiser, Philip and Kovacik, Lubomir and Engel, Andreas and Stahlberg, Henning and Palczewski, Krzysztof. (2020) Single particle cryo-EM of the complex between interphotoreceptor retinoid-binding protein and a monoclonal antibody. FASEB Journal, 34 (10). pp. 13918-13934.

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Abstract

Interphotoreceptor retinoid-binding protein (IRBP) is a highly expressed protein secreted by rod and cone photoreceptors that has major roles in photoreceptor homeostasis as well as retinoid and polyunsaturated fatty acid transport between the neural retina and retinal pigment epithelium. Despite two crystal structures reported on fragments of IRBP and decades of research, the overall structure of IRBP and function within the visual cycle remain unsolved. Here, we studied the structure of native bovine IRBP in complex with a monoclonal antibody (mAb5) by cryo-electron microscopy, revealing the tertiary and quaternary structure at sufficient resolution to clearly identify the complex components. Complementary mass spectrometry experiments revealed the structure and locations of N-linked carbohydrate post-translational modifications. This work provides insight into the structure of IRBP, displaying an elongated, flexible three-dimensional architecture not seen among other retinoid-binding proteins. This work is the first step in elucidation of the function of this enigmatic protein.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Federation of American Society of Experimental Biology
ISSN:0892-6638
e-ISSN:1530-6860
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:27 Jan 2022 13:58
Deposited On:27 Jan 2022 13:58

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