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Measuring the Metabolic Activity of Mature Mycobacterial Biofilms Using Isothermal Microcalorimetry

Solokhina, Anna and Bonkat, Gernot and Braissant, Olivier. (2019) Measuring the Metabolic Activity of Mature Mycobacterial Biofilms Using Isothermal Microcalorimetry. Methods in molecular biology (Clifton, N.J.), 1964. pp. 141-149.

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Official URL: https://edoc.unibas.ch/78923/

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Abstract

Measuring metabolic activity and response of biofilm to different conditions or compounds is of general interest but is also expected to help in developing new antibiofilm compounds and potentially new treatments. Current culture-based and microscopic methods although of much use have several drawbacks. Isothermal calorimetry can be useful in this context by allowing measurements of the metabolic activity of biofilm grown and maintained on solid medium. Biofilms prepared on membranes were placed in calorimetry vials containing solid medium. Sealed vials were introduced in an isothermal calorimeter, and the rate of metabolic heat production was monitored over time. We chose mycobacteria as an example for this paper as working with mycobacterial biofilms is notoriously difficult.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedical Engineering
UniBasel Contributors:Braissant, Olivier
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:1940-6029
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:03 Nov 2020 15:30
Deposited On:03 Nov 2020 15:30

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