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Phosphorylation Of MSI-1 Is Required For Forgetting In Caenorhabditis Elegans

Boglari, Csaba. Phosphorylation Of MSI-1 Is Required For Forgetting In Caenorhabditis Elegans. 2020, Doctoral Thesis, University of Basel, Faculty of Psychology.

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Official URL: https://edoc.unibas.ch/78745/

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Abstract

The Musashi family of RNA-binding proteins controls several biological processes including stem cell maintenance, cell division, and neural function. Previously, we demonstrated that the C. elegans Musashi ortholog, msi-1, regulates forgetting via translational repression of the Arp2/3 actin-branching complex. However, the mechanisms controlling MSI-1 activity during the regulation of forgetting are currently unknown. Here we investigated the effects of post-translational modifications on MSI-1 activity. We show that MSI-1 function is likely controlled by alterations of its activity rather than its expression levels. Furthermore, we found that MSI-1 is phosphorylated and identified the phosphorylation sites in the worm MSI-1 using mass spectrometry. Moreover, CRISPR-based manipulations of MSI-1 phosphorylation sites revealed that phosphorylation is necessary for MSI-1 function in both short- and long-term memory. Thus, our study provides a first insight into the controlling mechanism of MSI-1 activity during forgetting and may facilitate the development of novel therapeutic approaches.
Advisors:Papassotiropoulos, Andreas and de Quervain, Dominique J.-F.
Faculties and Departments:03 Faculty of Medicine > Bereich Psychiatrie (Klinik) > Erwachsenenpsychiatrie UPK > Molekulare Neurowissenschaften (Papassotiropoulos)
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Psychiatrie (Klinik) > Erwachsenenpsychiatrie UPK > Molekulare Neurowissenschaften (Papassotiropoulos)
05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Life Sciences Training Facility (Papassotiropoulos)
07 Faculty of Psychology > Departement Psychologie > Forschungsbereich Klinische Psychologie und Neurowissenschaften > Molecular Psychology (Papassotiropoulos)
UniBasel Contributors:Papassotiropoulos, Andreas and de Quervain, Dominique J.-F.
Item Type:Thesis
Thesis Subtype:Doctoral Thesis
Thesis no:13755
Thesis status:Complete
Number of Pages:40
Language:English
Identification Number:
  • urn: urn:nbn:ch:bel-bau-diss137559
edoc DOI:
Last Modified:04 Dec 2020 15:17
Deposited On:04 Dec 2020 15:17

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