Prepore Stability Controls Productive Folding of the BAM-independent Multimeric Outer Membrane Secretin PulD

Members of a group of multimeric secretion pores that assemble independently of any known membrane-embedded insertase in Gram-negative bacteria fold into a prepore before membrane-insertion occurs. The mechanisms and the energetics that drive the folding of these proteins are poorly understood. Here, equilibrium unfolding and hydrogen/deuterium exchange monitored by mass spectrometry indicated that a loss of 4-5 kJ/mol/protomer in the N-3 domain that is peripheral to the membrane-spanning C domain in the dodecameric secretin PulD, the founding member of this class, prevents pore formation by destabilizing the prepore into a poorly structured dodecamer as visualized by electron microscopy. Formation of native PulD-multimers by mixing protomers that differ in N-3 domain stability, suggested that the N-3 domain forms a thermodynamic seal onto the prepore. This highlights the role of modest free energy changes in the folding of preintegration forms of a hyperstable outer membrane complex and reveals a key driving force for assembly independently of the beta-barrel assembly machinery.