Cryo-electron tomography reveals novel features of a viral RNA replication compartment

Ertel, Kenneth J. and Benefield, Desiree and Castaño-Díez, Daniel and Pennington, Janice G. and Horswill, Mark and den Boon, Johan A. and Otegui, Marisa S. and Ahlquist, Paul . (2017) Cryo-electron tomography reveals novel features of a viral RNA replication compartment. eLife, 6. e25940.

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Official URL: https://edoc.unibas.ch/77898/

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Positive-strand RNA viruses, the largest genetic class of viruses, include numerous important pathogens such as Zika virus. These viruses replicate their RNA genomes in novel, membrane-bounded mini-organelles, but the organization of viral proteins and RNAs in these compartments has been largely unknown. We used cryo-electron tomography to reveal many previously unrecognized features of Flock house nodavirus (FHV) RNA replication compartments. These spherular invaginations of outer mitochondrial membranes are packed with electron-dense RNA fibrils and their volumes are closely correlated with RNA replication template length. Each spherule’s necked aperture is crowned by a striking cupped ring structure containing multifunctional FHV RNA replication protein A. Subtomogram averaging of these crowns revealed twelve-fold symmetry, concentric flanking protrusions, and a central electron density. Many crowns were associated with long cytoplasmic fibrils, likely to be exported progeny RNA. These results provide new mechanistic insights into positive-strand RNA virus replication compartment structure, assembly, function and control.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum
UniBasel Contributors:Castano Diez, Daniel
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:eLife Sciences Publications
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:21 Aug 2020 12:20
Deposited On:21 Aug 2020 12:20

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