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Shedding Light on Metal-Based Nanoparticles in Zebrafish by Computed Tomography with Micrometer Resolution

Cörek, Emre and Rodgers, Griffin and Siegrist, Stefan and Einfalt, Tomaz and Detampel, Pascal and Schlepütz, Christian M. and Sieber, Sandro and Fluder, Pascal and Schulz, Georg and Unterweger, Harald and Alexiou, Christoph and Müller, Bert and Puchkov, Maxim and Huwyler, Jörg. (2020) Shedding Light on Metal-Based Nanoparticles in Zebrafish by Computed Tomography with Micrometer Resolution. Small , 16 (31). p. 2000746.

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Official URL: https://edoc.unibas.ch/77507/

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Abstract

Metal‐based nanoparticles are clinically used for diagnostic and therapeutic applications. After parenteral administration, they will distribute throughout different organs. Quantification of their distribution within tissues in the 3D space, however, remains a challenge owing to the small particle diameter. In this study, synchrotron radiation‐based hard X‐ray tomography (SRμCT) in absorption and phase contrast modes is evaluated for the localization of superparamagnetic iron oxide nanoparticles (SPIONs) in soft tissues based on their electron density and X‐ray attenuation. Biodistribution of SPIONs is studied using zebrafish embryos as a vertebrate screening model. This label‐free approach gives rise to an isotropic, 3D, direct space visualization of the entire 2.5 mm‐long animal with a spatial resolution of around 2 µm. High resolution image stacks are available on a dedicated internet page ( http://zebrafish.pharma‐te.ch ). X‐ray tomography is combined with physico‐chemical characterization and cellular uptake studies to confirm the safety and effectiveness of protective SPION coatings. It is demonstrated that SRμCT provides unprecedented insights into the zebrafish embryo anatomy and tissue distribution of label‐free metal oxide nanoparticles.
Faculties and Departments:05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Pharmazie > Pharmazeutische Technologie (Huwyler)
UniBasel Contributors:Huwyler, Jörg and Cörek, Emre and Siegrist, Stefan and Einfalt, Tomaz and Detampel, Pascal and Sieber, Sandro and Müller, Bert and Rodgers, Griffin and Schulz, Georg and Puchkov, Maxim
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
e-ISSN:1613-6829
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:01 Nov 2021 14:17
Deposited On:01 Nov 2021 14:17

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