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Digital multiplex ligation assay for highly multiplexed screening of β-lactamase-encoding genes in bacterial isolates

Tamminen, Manu and Spaak, Jenny and Caduff, Lea and Schiff, Hanna and Lang, Ramon and Schmid, Steven and Montealegre, Maria Camila and Julian, Timothy R.. (2020) Digital multiplex ligation assay for highly multiplexed screening of β-lactamase-encoding genes in bacterial isolates. Communications biology, 3 (1). p. 264.

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Official URL: https://edoc.unibas.ch/76859/

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Abstract

Increasing incidence of antibiotic resistance in clinical and environmental settings calls for increased scalability in their surveillance. Current screening technologies are limited by the number of samples and genes that can easily be screened. We demonstrate here digital multiplex ligation assay (dMLA) as a low-cost targeted genomic detection workflow capable of highly-parallel screening of bacterial isolates for multiple target gene regions simultaneously. Here, dMLA is used for simultaneous detection of 1187 β-lactamase-encoding genes, including extended spectrum β-lactamase (ESBL) genes, in 74 bacterial isolates. We demonstrate dMLA as a light-weight and cost-efficient workflow which provides a highly scalable tool for antimicrobial resistance surveillance and is also adaptable to genetic screening applications beyond antibiotic resistance.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Eco System Health Sciences > Ecosystem Services, Climate & Health (Cissé)
UniBasel Contributors:Julian, Timothy
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Springer Nature
ISSN:2399-3642
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:16 Jun 2020 08:16
Deposited On:16 Jun 2020 08:16

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