Efficient Dual-Negative Selection for Bacterial Genome Editing

Cianfanelli, Francesca Romana and Cunrath, Olivier and Bumann, Dirk. (2020) Efficient Dual-Negative Selection for Bacterial Genome Editing.

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Official URL: https://edoc.unibas.ch/76528/

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We describe a versatile method for chromosomal gene editing based on classical consecutive single-crossovers. The method exploits rapid plasmid construction using Gibson assembly, a convenient E. coli donor strain, and efficient dual-negative selection for improved suicide vector resolution. We used this method to generate in frame deletions, insertions and point mutations in Salmonella enterica with limited hands-on time. Similar strategies allowed efficient gene editing also in Pseudomonas aeruginosa and multi-drug-resistant (MDR) Escherichia coli clinical isolates.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Bumann)
UniBasel Contributors:Bumann, Dirk
Item Type:Working Paper
Number of Pages:16
Note:Publication type according to Uni Basel Research Database: Discussion paper / Internet publication
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Last Modified:30 Dec 2020 09:31
Deposited On:04 May 2020 07:22

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