Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice

Wotzka, Sandra Y. and Kreuzer, Markus and Maier, Lisa and Arnoldini, Markus and Nguyen, Bidong D. and Brachmann, Alexander O. and Berthold, Dorothée L. and Zünd, Mirjam and Hausmann, Annika and Bakkeren, Erik and Hoces, Daniel and Gül, Ersin and Beutler, Markus and Dolowschiak, Tamas and Zimmermann, Michael and Fuhrer, Tobias and Moor, Kathrin and Sauer, Uwe and Typas, Athanasios and Piel, Jörn and Diard, Médéric and Macpherson, Andrew J. and Stecher, Bärbel and Sunagawa, Shinichi and Slack, Emma and Hardt, Wolf-Dietrich. (2019) Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice. Nature microbiology, 4 (12). pp. 2164-2174.

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Official URL: https://edoc.unibas.ch/76518/

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The microbiota confers colonization resistance, which blocks Salmonella gut colonization; 1; . As diet affects microbiota composition, we studied whether food composition shifts enhance susceptibility to infection. Shifting mice to diets with reduced fibre or elevated fat content for 24 h boosted Salmonella Typhimurium or Escherichia coli gut colonization and plasmid transfer. Here, we studied the effect of dietary fat. Colonization resistance was restored within 48 h of return to maintenance diet. Salmonella gut colonization was also boosted by two oral doses of oleic acid or bile salts. These pathogen blooms required Salmonella's AcrAB/TolC-dependent bile resistance. Our data indicate that fat-elicited bile promoted Salmonella gut colonization. Both E. coli and Salmonella show much higher bile resistance than the microbiota. Correspondingly, competitive E. coli can be protective in the fat-challenged gut. Diet shifts and fat-elicited bile promote S. Typhimurium gut infections in mice lacking E. coli in their microbiota. This mouse model may be useful for studying pathogen-microbiota-host interactions, the protective effect of E. coli, to analyse the spread of resistance plasmids and assess the impact of food components on the infection process.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Pathogen Evolution (Diard)
UniBasel Contributors:Diard, Médéric
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:21 May 2020 08:45
Deposited On:21 May 2020 08:45

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