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Restoring vision: therapy and mechanisms of disease

Nelidova, Dasha Elena. Restoring vision: therapy and mechanisms of disease. 2019, Doctoral Thesis, University of Basel, Faculty of Science.

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Official URL: http://edoc.unibas.ch/diss/DissB_13651

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Abstract

Enabling near-infrared light sensitivity in a blind human retina may supplement or restore visual function in patients with regional retinal degeneration. We induced near-infrared light sensitivity using gold nanorods bound to temperature-sensitive engineered transient receptor potential (TRP) channels. We expressed mammalian or snake TRP channels in light-insensitive retinal cones in a mouse model of retinal degeneration. Near-infrared stimulation increased activity in cones, ganglion cell layer neurons and cortical neurons, and enabled mice to perform a learned, light-driven behavior. We tuned responses to different wavelengths by using nanorods with different lengths, and to different radiant powers by using engineered channels with different temperature thresholds. We targeted TRP channels to human retinas, which allowed the activation of different cell types by near-infrared light, post-mortem.
Advisors:Roska, Botond and Mrsic-Flogel, Thomas
Faculties and Departments:09 Associated Institutions > Friedrich Miescher Institut FMI > Neurobiology > Structure and function of neural circuits (Roska)
UniBasel Contributors:Roska, Botond and Mrsic-Flogel, Thomas
Item Type:Thesis
Thesis Subtype:Doctoral Thesis
Thesis no:13651
Thesis status:Complete
Bibsysno:Link to catalogue
Number of Pages:1 Online-Ressource (79 Seiten)
Language:English
Identification Number:
Last Modified:01 Sep 2020 04:30
Deposited On:31 Aug 2020 11:54

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