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Molecular confirmation of a Fasciola gigantica x Fasciola hepatica hybrid in a Chadian bovine

Evack, Jennifer Giovanoli and Schmidt, Remo S. and Boltryk, Sylwia D. and Voss, Till S. and Batil, Annour A. and Ngandolo, Bongo N. and Greter, Helena and Utzinger, Jürg and Zinsstag, Jakob and Balmer, Oliver. (2020) Molecular confirmation of a Fasciola gigantica x Fasciola hepatica hybrid in a Chadian bovine. Journal of parasitology : official organ of the American Society of Parasitologists, 106 (2). pp. 316-322.

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Abstract

Fascioliasis is a zoonotic infection of humans and, more commonly, ruminants. It is caused by 2 liver fluke species, Fasciola hepatica and Fasciola gigantica, which differ in size. The traditional morphological methods used to distinguish the 2 species can be unreliable, particularly in the presence of hybrids between the 2 species. The development of advanced molecular methods has allowed for more definitive identification of Fasciola species, including their hybrids. Hybrids are of concern, as it is thought that they could acquire advantageous traits such as increased pathogenicity and host range. In 2013, we collected flukes from Fasciola-positive cattle, sheep, and goats slaughtered in 4 Chadian abattoirs. DNA from 27 flukes was extracted, amplified, and analyzed to identify species using the ITS1+2 locus. Twenty-six of the 27 flukes were identified as F. gigantica, while the remaining fluke showed heterozygosity at all variable sites that distinguish F. hepatica and F. gigantica. Cloning and sequencing of both alleles confirmed the presence of 1 F. hepatica and 1 F. gigantica allele. To our knowledge, this is the first unambiguous, molecular demonstration of the presence of such a hybrid in a bovine in sub-Saharan Africa.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Eco System Health Sciences > Health Impact Assessment (Winkler)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Human and Animal Health > One Health (Zinsstag)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Gene Regulation (Voss)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Parasite Chemotherapy (Mäser)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Swiss Centre for International Health (SCIH) > Health Systems Support (Prytherch)
UniBasel Contributors:Giovanoli Evack, Jennifer and Schmid, Reto and Voss, Till S and Greter, Helena and Utzinger, Jürg and Zinsstag, Jakob Z and Balmer, Oliver
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Society of Parasitologists
ISSN:0022-3395
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:08 May 2020 06:52
Deposited On:08 May 2020 06:52

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