edoc

Performance of nasal chondrocyte-based engineered tissues in osteoarthritis simulating environments

Acevedo Rua, Lina Marcela. Performance of nasal chondrocyte-based engineered tissues in osteoarthritis simulating environments. 2019, Doctoral Thesis, University of Basel, Faculty of Science.

[img]
Preview
PDF
10Mb

Official URL: http://edoc.unibas.ch/diss/DissB_13549

Downloads: Statistics Overview

Abstract

Osteoarthritis (OA) is the most prevalent musculoskeletal disease in humans, characterized by a progressive degeneration of the articulation that causes pain and disability in a large percentage of
the population. In this pathology, the joint environment becomes predominantly catabolic and
concentrations of circulating pro-inflammatory factors significantly increase as compared to nonpathological
conditions. Up to date, existing treatments can be effective in reduction of pain and
improvement of mobility, but none of the available therapies is able to stop the progression of the
disease.
Non-degenerative cartilaginous lesions can be currently treated with cell-based approaches,
consisting of the implantation of autologous chondrocytes into the defect site, those cells being
isolated from presumable non-affected areas of articular cartilage. Differentially, OA is considered
a contraindication for such treatments and, in the scarce cases they have been used for patients with
degenerative traces, failure is reported as the more common long-term outcome. Possible causes of
these results are the inferior chondrogenic capacity and phenotype stability demonstrated for
articular chondrocytes (AC) harvested from affected joints, but also, the detrimental conditions of
the OA environment, potentially compromising the performance of any implanted cell-based
product.
Nasal chondrocytes (NC) represent an alternative source for cell and tissue engineering approaches,
since they can be obtained from a compartment that is not affected (i.e., the nasal septum), and
show more reproducible capacity to generate functional cartilaginous tissues as well as similar
responses to mechanical and inflammatory stimuli than AC. In fact, tissue engineered cartilage
derived from nasal chondrocytes (N-TEC) have been already used in the clinic for the treatment of
post-traumatic cartilage lesions, but not results are generated regarding their potential to
additionally treat OA defects. In order to assess such potential, it is necessary to evaluate if N-TEC
can survive and maintain their tissue-like properties in the pro-inflammatory and catabolic OA
environment, to which cells from the different joint tissues (cartilage, synovial membrane and
subchondral bone) contribute.
The pillar of this thesis, my PhD dissertation, consists on the exploration of the suitability of NTEC
for the treatment of OA lesions. Therefore, this manuscript summarizes methods and
outcomes resulting from investigating the interactions between nasal chondrocytes and cells/tissues
from OA-joints, as an approach to establish the possible compatibility of N-TEC within an OA
cartilage defect. Results showed that N-TEC could maintain their cartilaginous properties, when
exposed in vitro to inflammatory stimuli as those found in OA joints, and positively influence the
inflammatory profile of cells from OA joints through secreted factors. Moreover, N-TEC were able
to survive and engraft into OA compartments simulated in vivo, while preserving cartilaginous
matrix properties and dampening inflammation, as observed in vitro.
Acknowledging the positive and wide compatibility of N-TEC within OA environments that I
demonstrated, the clinical application of autologous N-TEC was tested in two patients with
advanced OA, who would have been otherwise considered for partial knee – prosthetic -
replacement. After 14 months of implantation, patients have reported reduced pain as well as
improved joint function and life quality; all findings indicating that N-TEC can be envisioned as a
therapeutic approach for the repair of osteoarthritic knee cartilage defects. To assess efficacy of
this procedure, a phase II trial would be required in a larger cohort of patients.
Advisors:Martin, Ivan and Handschin, Christoph and Neutzner, Albert
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
05 Faculty of Science
UniBasel Contributors:Martin, Ivan and Handschin, Christoph and Neutzner, Albert
Item Type:Thesis
Thesis Subtype:Doctoral Thesis
Thesis no:13549
Thesis status:Complete
Bibsysno:Link to catalogue
Number of Pages:1 Online-Ressource (146 Seiten)
Language:English
Identification Number:
Last Modified:06 May 2020 04:30
Deposited On:05 May 2020 12:04

Repository Staff Only: item control page