Methods used in the structure determination of bovine mitochondrial F-1 ATPase

Abrahams, J. P. and Leslie, A. G. W.. (1996) Methods used in the structure determination of bovine mitochondrial F-1 ATPase. Acta Crystallographica. Section D, Biological Crystallography, 52. pp. 30-42.

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With a size of 372 kDa, the F-1 ATPase particle is the largest asymmetric structure solved to date. Isomorphous differences arising from reacting the crystals with methyl-mercury nitrate at two concentrations allowed the structure determination, Careful data collection and data processing were essential in this process as well as a new form of electron-density modification, 'solvent flipping'. The most important feature of this new procedure is that the electron density in the solvent region is inverted rather than set to a constant value, as in conventional solvent flattening. All non-standard techniques and variations on new techniques which were employed in the structure determination are described.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nano-diffraction of Biological Specimen (Abrahams)
UniBasel Contributors:Abrahams, Jan Pieter
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:05 Nov 2020 14:47
Deposited On:05 Nov 2020 14:47

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