Screening crystallisation conditions using fluorescence correlation spectroscopy

Schmauder, R. and Schmidt, T. and Abrahams, J. P. and Kuil, M. E.. (2002) Screening crystallisation conditions using fluorescence correlation spectroscopy. Acta Crystallographica Section D: Structural Biology, 58. pp. 1536-1541.

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We investigate the potential of fluorescence correlation spectroscopy (FCS) in screening for crystallisation conditions. Solutions that nucleate protein crystals must have different interactions than solutions that do not give rise to crystals. Due to these different interactions the average mean squared displacement of the individual proteins changes. By monitoring protein self-diffusion, we can distinguish crystallising from non-crystallising solutions. The method introduced can be applied at extremely low concentrations in femtoliter volumes as an early diagnostic for molecular association. Based on our preliminary findings FCS has the potential to become a routine screening method for crystallography.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nano-diffraction of Biological Specimen (Abrahams)
UniBasel Contributors:Abrahams, Jan Pieter
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:International Union of Crystallography
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:05 Sep 2023 08:35
Deposited On:05 Nov 2020 15:23

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