A novel approximation method of CTF amplitude correction for 3D single particle reconstruction

Jiang, Linhua and Liu, Zunfeng and Georgieva, Dilyana and Kuil, Maxim E. and Abrahams, Jan Pieter. (2010) A novel approximation method of CTF amplitude correction for 3D single particle reconstruction. Ultramicroscopy, 110 (4). pp. 350-358.

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Official URL: https://edoc.unibas.ch/75922/

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The typical resolution of three-dimensional reconstruction by cryo-EM single particle analysis is now being pushed up to and beyond the nanometer scale. Correction of the contrast transfer function (CTF) of electron microscopic images is essential for achieving such a high resolution. Various correction methods exist and are employed in popular reconstruction software packages. Here, we present a novel approximation method that corrects the amplitude modulation introduced by the contrast transfer function by convoluting the images with a piecewise continuous function. Our new approach can easily be implemented and incorporated into other packages. The implemented method yielded higher resolution reconstructions with data sets from both highly symmetric and asymmetric structures. It is an efficient alternative correction method that allows quick convergence of the 3D reconstruction and has a high tolerance for noisy images, thus easing a bottleneck in practical reconstruction of macromolecules.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nano-diffraction of Biological Specimen (Abrahams)
UniBasel Contributors:Abrahams, Jan Pieter
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:06 Nov 2020 08:01
Deposited On:06 Nov 2020 08:01

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