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A helical inner scaffold provides a structural basis for centriole cohesion

Le Guennec, Maeva and Klena, Nikolai and Gambarotto, Davide and Laporte, Marine H. and Tassin, Anne-Marie and van den Hoek, Hugo and Erdmann, Philipp S. and Schaffer, Miroslava and Kovacik, Lubomir and Borgers, Susanne and Goldie, Kenneth N. and Stahlberg, Henning and Bornens, Michel and Azimzadeh, Juliette and Engel, Benjamin D. and Hamel, Virginie and Guichard, Paul. (2020) A helical inner scaffold provides a structural basis for centriole cohesion. Science Advances, 6 (7). eaaz4137.

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Official URL: https://edoc.unibas.ch/75796/

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Abstract

The ninefold radial arrangement of microtubule triplets (MTTs) is the hallmark of the centriole, a conserved organelle crucial for the formation of centrosomes and cilia. Although strong cohesion between MTTs is critical to resist forces applied by ciliary beating and the mitotic spindle, how the centriole maintains its structural integrity is not known. Using cryo-electron tomography and subtomogram averaging of centrioles from four evolutionarily distant species, we found that MTTs are bound together by a helical inner scaffold covering ~70% of the centriole length that maintains MTTs cohesion under compressive forces. Ultrastructure Expansion Microscopy (U-ExM) indicated that POC5, POC1B, FAM161A, and Centrin-2 localize to the scaffold structure along the inner wall of the centriole MTTs. Moreover, we established that these four proteins interact with each other to form a complex that binds microtubules. Together, our results provide a structural and molecular basis for centriole cohesion and geometry.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Association for the Advancement of Science
ISSN:2375-2548
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:23 Nov 2021 16:36
Deposited On:23 Nov 2021 16:36

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