edoc

Mechanically defined microenvironment promotes stabilization of microvasculature, which correlates with the enrichment of a novel Piezo-1+ population of circulating CD11b+/CD115+ monocytes

Forget, Aurelien and Gianni-Barrera, Roberto and Uccelli, Andrea and Sarem, Melika and Kohler, Esther and Fogli, Barbara and Muraro, Manuele Giuseppe and Bichet, Sandrine and Aumann, Konrad and Banfi, Andrea and Shastri, Vera Prasad. (2019) Mechanically defined microenvironment promotes stabilization of microvasculature, which correlates with the enrichment of a novel Piezo-1+ population of circulating CD11b+/CD115+ monocytes. Advanced materials, 31 (21). e1808050.

[img] PDF - Accepted Version
6Mb

Official URL: https://edoc.unibas.ch/75711/

Downloads: Statistics Overview

Abstract

Vascularization is a critical step in the restoration of cellular homeostasis. Several strategies including localized growth factor delivery, endothelial progenitor cells, genetically engineered cells, gene therapy, and prevascularized implants have been explored to promote revascularization. But, long-term stabilization of newly induced vessels remains a challenge. It has been shown that fibroblasts and mesenchymal stem cells can stabilize newly induced vessels. However, whether an injected biomaterial alone can serve as an instructive environment for angiogenesis remains to be elucidated. It is reported here that appropriate vascular branching, and long-term stabilization can be promoted simply by implanting a hydrogel with stiffness matching that of fibrin clot. A unique subpopulation of circulating CD11b+ myeloid and CD11b+ /CD115+ monocytes that express the stretch activated cation channel Piezo-1, which is enriched prominently in the clot-like hydrogel, is identified. These findings offer evidence for a mechanobiology paradigm in angiogenesis involving an interplay between mechanosensitive circulating cells and mechanics of tissue microenvironment.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Cell and Gene Therapy (Banfi)
UniBasel Contributors:Banfi, Andrea and Gianni-Barrera, Roberto and Uccelli, Andrea and Muraro, Manuele Giuseppe
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:0935-9648
e-ISSN:1521-4095
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
edoc DOI:
Last Modified:03 Nov 2020 08:00
Deposited On:20 Aug 2020 14:40

Repository Staff Only: item control page