Targeted INO80 enhances subnuclear chromatin movement and ectopic homologous recombination

Neumann, Frank R. and Dion, Vincent and Gehlen, Lutz R. and Tsai-Pflugfelder, Monika and Schmid, Roger and Taddei, Angela and Gasser, Susan M.. (2012) Targeted INO80 enhances subnuclear chromatin movement and ectopic homologous recombination. Genes and Development, 26 (4). pp. 369-383.

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Official URL: https://edoc.unibas.ch/75464/

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Chromatin in the interphase nucleus moves in a constrained random walk. Despite extensive study, the molecular causes of such movement and its impact on DNA-based reactions are unclear. Using high-precision live fluorescence microscopy in budding yeast, we quantified the movement of tagged chromosomal loci to which transcriptional activators or nucleosome remodeling complexes were targeted. We found that local binding of the transcriptional activator VP16, but not of the Gal4 acidic domain, enhances chromatin mobility. The increase in movement did not correlate strictly with RNA polymerase II (PolII) elongation, but could be phenocopied by targeting the INO80 remodeler to the locus. Enhanced chromatin mobility required Ino80's ATPase activity. Consistently, the INO80-dependent remodeling of nucleosomes upon transcriptional activation of the endogenous PHO5 promoter enhanced chromatin movement locally. Finally, increased mobility at a double-strand break was also shown to depend in part on the INO80 complex. This correlated with increased rates of spontaneous gene conversion. We propose that local chromatin remodeling and nucleosome eviction increase large-scale chromatin movements by enhancing the flexibility of the chromatin fiber.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin
UniBasel Contributors:Neumann, Frank R.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cold Spring Harbor Laboratory Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:01 Oct 2020 07:03
Deposited On:01 Oct 2020 07:03

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