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Differential scaling between G1 protein production and cell size dynamics promotes commitment to the cell division cycle in budding yeast

Litsios, Athanasios and Huberts, Daphne H. E. W. and Terpstra, Hanna M. and Guerra, Paolo and Schmidt, Alexander and Buczak, Katarzyna and Papagiannakis, Alexandros and Rovetta, Mattia and Hekelaar, Johan and Hubmann, Georg and Exterkate, Marten and Milias-Argeitis, Andreas and Heinemann, Matthias. (2019) Differential scaling between G1 protein production and cell size dynamics promotes commitment to the cell division cycle in budding yeast. Nature cell biology, 21 (11). pp. 1382-1392.

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Official URL: https://edoc.unibas.ch/75073/

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Abstract

In the unicellular eukaryote Saccharomyces cerevisiae, Cln3-cyclin-dependent kinase activity enables Start, the irreversible commitment to the cell division cycle. However, the concentration of Cln3 has been paradoxically considered to remain constant during G1, due to the presumed scaling of its production rate with cell size dynamics. Measuring metabolic and biosynthetic activity during cell cycle progression in single cells, we found that cells exhibit pulses in their protein production rate. Rather than scaling with cell size dynamics, these pulses follow the intrinsic metabolic dynamics, peaking around Start. Using a viral-based bicistronic construct and targeted proteomics to measure Cln3 at the single-cell and population levels, we show that the differential scaling between protein production and cell size leads to a temporal increase in Cln3 concentration, and passage through Start. This differential scaling causes Start in both daughter and mother cells across growth conditions. Thus, uncoupling between two fundamental physiological parameters drives cell cycle commitment.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Proteomics (Schmidt)
UniBasel Contributors:Schmidt, Alexander
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:1476-4679
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:05 Apr 2020 17:10
Deposited On:05 Apr 2020 17:10

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