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Differential regulation of germline mRNAs in soma and germ cells by zebrafish miR-430

Mishima, Yuichiro and Giraldez, Antonio J. and Takeda, Yasuaki and Fujiwara, Toshinobu and Sakamoto, Hiroshi and Schier, Alexander F. and Inoue, Kunio. (2006) Differential regulation of germline mRNAs in soma and germ cells by zebrafish miR-430. Current Biology, 16 (21). pp. 2135-2142.

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Official URL: https://edoc.unibas.ch/74864/

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Abstract

Early in development, primordial germ cells (PGCs) are set aside from somatic cells and acquire a unique gene-expression program . The mechanisms underlying germline-specific gene expression are largely unknown. Nanos expression is required during germline development and is posttranscriptionally restricted to PGCs . Here we report that the microRNA miR-430 targets the 3' untranslated region (UTR) of nanos1 during zebrafish embryogenesis. A miR-430 target site within the nanos1 3' UTR reduces poly(A) tail length, mRNA stability, and translation. Repression is disrupted in maternal-zygotic dicer mutants (MZdicer), which lack mature miRNAs , and is restored by injection of processed miR-430. Although miR-430 represses other genes equally in germline and soma, specific regions in the nanos1 3' UTR compensate for microRNA-mediated repression in PGCs and allow germline-specific expression. We show that the 3' UTR of an additional PGC-specific gene, TDRD7, is also targeted by miR-430. These results indicate that miR-430 targets the 3' UTRs of germline genes and suggest that differential susceptibility to microRNAs contributes to tissue-specific gene expression.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Schier)
UniBasel Contributors:Schier, Alexander F
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cell Press
ISSN:0960-9822
e-ISSN:1879-0445
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:18 Dec 2020 14:47
Deposited On:18 Dec 2020 14:47

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