Choi, Wen-Yee and Giraldez, Antonio J. and Schier, Alexander F.. (2007) Target protectors reveal dampening and balancing of Nodal agonist and antagonist by miR-430. Science, 318 (5848). pp. 271-274.
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Official URL: https://edoc.unibas.ch/74860/
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Abstract
MicroRNAs (miRNAs) repress hundreds of target messenger RNAs (mRNAs), but the physiological roles of specific miRNA-mRNA interactions remain largely elusive. We report that zebrafish microRNA-430 (miR-430) dampens and balances the expression of the transforming growth factor-beta (TGF-beta) Nodal agonist squint and the TGF-beta Nodal antagonist lefty. To disrupt the interaction of specific miRNA-mRNA pairs, we developed target protector morpholinos complementary to miRNA binding sites in target mRNAs. Protection of squint or lefty mRNAs from miR-430 resulted in enhanced or reduced Nodal signaling, respectively. Simultaneous protection of squint and lefty or absence of miR-430 caused an imbalance and reduction in Nodal signaling. These findings establish an approach to analyze the in vivo roles of specific miRNA-mRNA pairs and reveal a requirement for miRNAs in dampening and balancing agonist/antagonist pairs.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Schier) |
|---|---|
| UniBasel Contributors: | Schier, Alexander F |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | American Association for the Advancement of Science |
| ISSN: | 0036-8075 |
| e-ISSN: | 1095-9203 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Identification Number: |
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| Last Modified: | 18 Dec 2020 14:59 |
| Deposited On: | 18 Dec 2020 14:59 |
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