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Bi-zonal cartilaginous tissues engineered in a rotary cell culture system

Marsano, A. and Wendt, D. and Quinn, T. M. and Sims, T. J. and Farhadi, J. and Jakob, M. and Heberer, M. and Martin, I.. (2006) Bi-zonal cartilaginous tissues engineered in a rotary cell culture system. Biorheology, Vol. 43, H. 3-4. pp. 553-560.

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Official URL: http://edoc.unibas.ch/dok/A5249017

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Abstract

In this study, we aimed at validating a rotary cell culture system (RCCS) bioreactor with medium recirculation and external oxygenation, for cartilage tissue engineering. Primary bovine and human culture-expanded chondrocytes were seeded into non-woven meshes of esterified hyaluronan (HYAFF((R))-11), and the resulting constructs were cultured statically or in the RCCS, in the presence of insulin and TGF beta 3, for up to 4 weeks. Culture in the RCCS did not induce significant differences in the contents of glycosaminoglycans (GAG) and collagen deposited, but markedly affected their distribution. In contrast to statically grown tissues, engineered cartilage cultured in the RCCS had a bi-zonal structure, consisting of an outgrowing fibrous capsule deficient in GAG and rich in collagen, and an inner region more positively stained for GAG. Structurally, trends were similar using primary bovine or expanded human chondrocytes, although the human cells deposited inferior amounts of matrix. The use of the presented RCCS, in conjunction with the described medium composition, has the potential to generate bi-zonal tissues with features qualitatively resembling the native meniscus.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
UniBasel Contributors:Martin, Ivan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:IOS Press
ISSN:0006-355X
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:31 Dec 2015 10:44
Deposited On:22 Mar 2012 13:39

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