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Precultivation of engineered human nasal cartilage enhances the mechanical properties relevant for use in facial reconstructive surgery

Farhadi, Jian and Fulco, Ilario and Miot, Sylvie and Wirz, Dieter and Haug, Martin and Dickinson, Sally C. and Hollander, Anthony P. and Daniels, A. U. and Pierer, Gerhard and Heberer, Michael and Martin, Ivan. (2006) Precultivation of engineered human nasal cartilage enhances the mechanical properties relevant for use in facial reconstructive surgery. Annals of surgery, Vol. 244, Nr. 6. pp. 978-985.

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Official URL: http://edoc.unibas.ch/dok/A5249006

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Abstract

Objective: To investigate if precultivation of human engineered nasal cartilage grafts of clinically relevant size would increase the suture retention strength at implantation and the tensile and bending stiffness 2 weeks after implantation. Summary Background Information: To be used for reconstruction of nasal cartilage defects, engineered grafts need to be reliably sutured at implantation and resist to bending/tension forces about 2 weeks after surgery, when fixation is typically removed. Methods: Nasal septum chondrocytes from 4 donors were expanded for 2 passages and statically loaded on 15 X 5 X 2-mm size nonwoven meshes of esterified hyaluronan (Hyaff-11). Constructs were implanted for 2 weeks in nude mice between muscle fascia and subcutaneous tissue either directly after cell seeding or after 2 or 4 weeks of preculture in chondrogenic medium. Engineered tissues and native nasal cartilage were assessed histologically, biochemically, and biomechanically. Results: Engineered constructs reproducibly developed with culture time into cartilaginous tissues with increasing content of glycosaminoglycans and collagen type II. Suture retention strength was significantly higher (3.6 +/- 2.2-fold) in 2-week precultured constructs than in freshly seeded meshes. Following in vivo implantation, tissues further developed and maintained the original scaffold size and shape. The bending stiffness was significantly higher (1.8 +/- 0.8-fold) if constructs were precultured for 2 weeks than if they were directly implanted, whereas tensile stiffness was close to native cartilage in all groups. Conclusion: In our experimental setup, preculture for 2 weeks was necessary to engineer nasal cartilage grafts with enhanced mechanical properties relevant for clinical use in facial reconstructive surgery.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
UniBasel Contributors:Martin, Ivan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Lippincott Williams & Wilkins
ISSN:0003-4932
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:31 Dec 2015 10:44
Deposited On:22 Mar 2012 13:39

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