Human articular chondrocytes suppress in vitro proliferation of anti-CD3 activated peripheral blood mononuclear cells

Bocelli-Tyndall, Chiara and Barbero, Andrea and Candrian, Christian and Ceredig, Rhodri and Tyndall, Alan and Martin, Ivan. (2006) Human articular chondrocytes suppress in vitro proliferation of anti-CD3 activated peripheral blood mononuclear cells. Journal of cellular physiology, Vol. 209, no. 3. pp. 732-734.

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Official URL: http://edoc.unibas.ch/dok/A5248923

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Objective: To investigate whether mature human articular chondrocytes (AC) exhibit an anti proliferative effect on activated peripheral blood mononuclear cells (PBMC) and to compare this effect with other cells of mesenchymal origin. Methods: AC from healthy cadaveric cartilage were grown for different passages, in the absence (control) or presence of factors enhancing cell dedifferentiation (transforming growth factor (TGF)beta 1, fibroblast growth factor (FGF)-2, and platelet derived growth factor (PDGF)bb-TFP medium). Cell ability to suppress PBMC proliferation driven by anti-CD3 antibody was measured by tritiated thymidine uptake following incubation for 48 h at different PBMC:AC ratios and expressed as percent of residual proliferation (RP) AC anti proliferative effect was compared to that of control dermal fibroblasts (DF) and bone marrow stromal cells (BMSC). Results: AC exhibited a cell number-dependent anti proliferative effect. The strongest effect (up to 2 TFP medium for AC expansion resulted in a significantly lower anti proliferative effect, in the range of that induced by BMSC (up to 18 RP). Also DF induced a marked anti proliferative effect (up to 11 Conclusion: We report for the first time that human AC have a marked anti proliferative effect on anti-CD3 stimulated PBMC, which is reduced upon culture in medium-inducing extensive cell de-differentiation. These results reflect the immunosuppressive properties observed for other different mesenchymal cell types and raise the question of a potential common physiological role in local tissue protection.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
UniBasel Contributors:Martin, Ivan
Item Type:Article, refereed
Article Subtype:Book Review
Publisher:Wiley Liss
Note:Publication type according to Uni Basel Research Database: Journal item
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Last Modified:22 Mar 2012 14:24
Deposited On:22 Mar 2012 13:39

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