Effects of fluid flow and calcium phosphate coating on human bone marrow stromal cells cultured in a defined 2D model system

Scaglione, S. and Wendt, D. and Miggino, S. and Papadimitropoulos, A. and Fato, M. and Quarto, R. and Martin, I.. (2008) Effects of fluid flow and calcium phosphate coating on human bone marrow stromal cells cultured in a defined 2D model system. Journal of biomedical materials research. Part A, 86 (2). pp. 411-419.

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In this study, we investigated the effect of the long-term (10 days) application of a defined and uniform level of fluid flow (uniform shear stress of 1.2 x 10(-3) N/m(2)) on human bone marrow stromal cells (BMSC) cultured on different substrates (i.e., uncoated glass or calcium phosphate coated glass, Osteologic(TM)) in a 2D parallel plate model. Both exposure to flow and culture on Osteologic significantly reduced the number of cell doublings. BMSC cultured under flow were more intensely stained for collagen type I and by von Kossa for mineralized matrix. BMSC exposed to flow displayed an increased osteogenic commitment (i.e., higher mRNA expression of cbfa-1 and osterix), although phenotype changes in response to flow (i.e., mRNA expression of osteopontin, osteocalcin and bone sialoprotein) were dependent on the substrate used. These findings highlight the importance of the combination of physical forces and culture substrate to determine the functional state of differentiating osteoblastic cells. The results obtained using a simple and controlled 2D model system may help to interpret the long-term effects of BMSC culture under perfusion within 3D porous scaffolds, where multiple experimental variables cannot be easily studied independently, and shear stresses cannot be precisely computed.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
UniBasel Contributors:Martin, Ivan
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Interscience Publishers
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:03 Oct 2017 08:51
Deposited On:22 Mar 2012 13:39

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