Sucrase-isomaltase and cystic fibrosis

Sips, H. J. and Claass, A. H. and van Dongen, J. M. and Willemsen, R. and Hoogeveen, A. T. and Galjaard, H. and Sinaasappel, M. and Hauri, H. P. and Sterchi, E. E.. (1985) Sucrase-isomaltase and cystic fibrosis. Journal of inherited metabolic disease, Vol. 8, no. 4. pp. 163-168.

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Official URL: http://edoc.unibas.ch/dok/A5257841

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The intestinal microvillar enzyme complex sucrase-isomaltase has been studied in cystic fibrosis and control ileum. A number of biochemical parameters of the enzyme in ileum homogenates have been determined. Both solubilized as well as membrane-bound sucrase-isomaltase were analyzed with respect to their reaction with monoclonal antibodies against human sucrase-isomaltase. Finally the subcellular localization of sucrase-isomaltase was verified by immunoelectronmicroscopy or via the analysis of purified brush-border membrane preparations. At all levels no significant differences could be detected between sucrase-isomaltase of cystic fibrosis and control ileum. It is concluded that an abnormal subcellular localization and/or abnormal enzymatic activity of sucrase-isomaltase in cystic fibrosis intestine cannot explain the markedly decreased disaccharidase activities in amniotic fluids from pregnancies resulting in a child affected with cystic fibrosis.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Pharmacology/Neurobiology (Hauri)
UniBasel Contributors:Hauri, Hans-Peter
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:24
Deposited On:22 Mar 2012 13:39

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