Probing the dynamic stalk region of the ribosome using solution NMR

Wang, Xiaolin and Kirkpatrick, John P. and Launay, Hélène M. M. and de Simone, Alfonso and Häussinger, Daniel and Dobson, Christopher M. and Vendruscolo, Michele and Cabrita, Lisa D. and Waudby, Christopher A. and Christodoulou, John. (2019) Probing the dynamic stalk region of the ribosome using solution NMR. Scientific reports, 9 (1). p. 13528.

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We describe an NMR approach based on the measurement of residual dipolar couplings (RDCs) to probe the structural and motional properties of the dynamic regions of the ribosome. Alignment of intact 70S ribosomes in filamentous bacteriophage enabled measurement of RDCs in the mobile C-terminal domain (CTD) of the stalk protein bL12. A structural refinement of this domain using the observed RDCs did not show large changes relative to the isolated protein in the absence of the ribosome, and we also found that alignment of the CTD was almost independent of the presence of the core ribosome particle, indicating that the inter-domain linker has significant flexibility. The nature of this linker was subsequently probed in more detail using a paramagnetic alignment strategy, which revealed partial propagation of alignment between neighbouring domains, providing direct experimental validation of a structural ensemble previously derived from SAXS and NMR relaxation measurements. Our results demonstrate the prospect of better characterising dynamical and functional regions of more challenging macromolecular machines and systems, for example ribosome-nascent chain complexes.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Nuclear Magnetic Resonance (Häussinger)
UniBasel Contributors:Häussinger, Daniel
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:04 Feb 2020 11:57
Deposited On:04 Feb 2020 11:57

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