edoc

Chemo-enzymatic cascades to produce cycloalkenes from bio-based resources

Wu, Shuke and Zhou, Yi and Gerngross, Daniel and Jeschek, Markus and Ward, Thomas R.. (2019) Chemo-enzymatic cascades to produce cycloalkenes from bio-based resources. Nature Communications, 10. p. 5060.

[img] PDF - Published Version
Available under License CC BY (Attribution).

1099Kb

Official URL: https://edoc.unibas.ch/73669/

Downloads: Statistics Overview

Abstract

Engineered enzyme cascades offer powerful tools to convert renewable resources into value-added products. Man-made catalysts give access to new-to-nature reactivities that may complement the enzyme's repertoire. Their mutual incompatibility, however, challenges their integration into concurrent chemo-enzymatic cascades. Herein we show that compartmentalization of complex enzyme cascades within E. coli whole cells enables the simultaneous use of a metathesis catalyst, thus allowing the sustainable one-pot production of cycloalkenes from oleic acid. Cycloheptene is produced from oleic acid via a concurrent enzymatic oxidative decarboxylation and ring-closing metathesis. Cyclohexene and cyclopentene are produced from oleic acid via either a six- or eight-step enzyme cascade involving hydration, oxidation, hydrolysis and decarboxylation, followed by ring-closing metathesis. Integration of an upstream hydrolase enables the usage of olive oil as the substrate for the production of cycloalkenes. This work highlights the potential of integrating organometallic catalysis with whole-cell enzyme cascades of high complexity to enable sustainable chemistry.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Bioanorganische Chemie (Ward)
UniBasel Contributors:Ward, Thomas R. R.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
ISSN:2041-1723
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:04 Feb 2020 10:57
Deposited On:04 Feb 2020 10:57

Repository Staff Only: item control page