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Porphyrin Containing Polymersomes with Enhanced ROS Generation Efficiency: in vitro evaluation

Kyropoulou, Myrto and DiLeone, Stefano and Lanzilotto, Angelo and Constable, Edwin C. and Housecroft, Catherine E. and Meier, Wolfgang P. and Palivan, Cornelia G.. (2019) Porphyrin Containing Polymersomes with Enhanced ROS Generation Efficiency: in vitro evaluation. Macromolecular Bioscience, 20 (2). p. 1900291.

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Abstract

Abstract Porphyrins are molecules possessing unique photophysical properties making them suitable for application in photodynamic therapy. The incorporation of porphyrins into natural or synthetic nano-assemblies such as polymersomes is a strategy to improve and prolong their therapeutic capacities and to overcome their limitations as therapeutic and diagnostic agents. Here, 5,10,15,20-tetrakis(1-(6-ethoxy-6-oxohexyl)-4-pyridin-1-io)-21H,23H-porphyrin tetrabromide porphyrin is inserted into polymersomes in order to demonstrate that the encapsulation enhances its ability to generate highly reactive singlet oxygen (1O2) upon irradiation in vitro. The photoactivation of the free and polymersome-encapsulated porphyrin is evaluated by electron spin resonance and cell viability assays on three different mammalian cell lines. The results indicate that by encapsulating the porphyrin, a controlled ROS delivery within the cells is achieved, at the same time avoiding side effects such as dark toxicity, non-specific porphyrin release and over time decreased activity in vitro. This work focuses on showing a not-toxic model system for modern therapeutic nanomedicine, which works under mild irradiation and dosage conditions.
Faculties and Departments:05 Faculty of Science > Departement Chemie
05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)
UniBasel Contributors:Meier, Wolfgang P.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:1616-5187
e-ISSN:1616-5195
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:10 Mar 2020 07:22
Deposited On:21 Jan 2020 10:59

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