Individual long non-coding RNAs have no overt functions in zebrafish embryogenesis, viability and fertility

Goudarzi, Mehdi and Berg, Kathryn and Pieper, Lindsey M. and Schier, Alexander F.. (2019) Individual long non-coding RNAs have no overt functions in zebrafish embryogenesis, viability and fertility. eLife, 8. 8:e40815.

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Official URL: https://edoc.unibas.ch/72734/

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Hundreds of long non-coding RNAs (lncRNAs) have been identified as potential regulators of gene expression, but their functions remain largely unknown. To study the role of lncRNAs during vertebrate development, we selected 25 zebrafish lncRNAs based on their conservation, expression profile or proximity to developmental regulators, and used CRISPR-Cas9 to generate 32 deletion alleles. We observed altered transcription of neighboring genes in some mutants, but none of the lncRNAs were required for embryogenesis, viability or fertility. Even RNAs with previously proposed non-coding functions (; cyrano; and; squint; ) and other conserved lncRNAs (; gas5; and; lnc-setd1ba); were dispensable. In one case (; lnc-phox2bb; ), absence of putative DNA regulatory-elements, but not of the lncRNA transcript itself, resulted in abnormal development. LncRNAs might have redundant, subtle, or context-dependent roles, but extrapolation from our results suggests that the majority of individual zebrafish lncRNAs have no overt roles in embryogenesis, viability and fertility.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Schier)
UniBasel Contributors:Schier, Alexander
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:eLife Sciences Publications
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:20 Aug 2020 14:14
Deposited On:20 Aug 2020 14:14

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