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Prism-based multicolor fluorescence correlation spectrometer

Chin Hwang, Ling and Leutenegger, Marcel and Gösch, Michael and Lasser, Theo and Rigler, Per and Meier, Wolfgang and Wohland, Thorsten . (2006) Prism-based multicolor fluorescence correlation spectrometer. Optics Letters, 31 (9). pp. 1310-1312.

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Official URL: https://edoc.unibas.ch/72417/

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Abstract

We report the design and application of a prism-based detection system for fluorescence (cross) correlation spectroscopy. The system utilizes a single laser wavelength for the simultaneous excitation of several dyes of different emission spectra. Fluorescence light is spectrally separated with a prismatic setup, and wavelengths are selected by scanning a fiber-coupled avalanche photodiode across the image spots. Multicolor autocorrelations are demonstrated with standard and tandem dyes, and fluorescence cross-correlation measurements of biotinylated nanocontainers and streptavidin are presented. This spectrometer offers high optical stability and no focal volume mismatch for the multicolor detection of molecular dynamics and interactions, with single-molecule sensitivity.
Faculties and Departments:05 Faculty of Science > Departement Chemie
05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)
UniBasel Contributors:Meier, Wolfgang P.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Optical Society of America
ISSN:0146-9592
e-ISSN:1539-4794
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:18 Mar 2020 14:28
Deposited On:18 Mar 2020 14:28

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