A Nanocompartment system (Synthosome) Designed for Biotechnical Applications

Nallani, Madhavan and Benito, Samantha Mariela and Onaca-Fischer, Ozana and Graff, Alexandra and Lindemann, Marcus and Winterhalter, Mathias and Meier, Wolfgang and Schwaneberg, Ulrich. (2006) A Nanocompartment system (Synthosome) Designed for Biotechnical Applications. Journal of Biotechnology, 123 (1). pp. 50-59.

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Official URL: https://edoc.unibas.ch/72416/

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A nanocompartment system based on two deletion mutants of the large channel protein FhuA (FhuA Delta 1-129; FhuA Delta 1-160) and an ABA triblock copolymer (PMOXA-PDMS-PMOXA) has been developed for putative biotechnological applications. FhuA is ideally suited for applications in biotechnology due to its monomeric structure, large pore diameter (39-46 angstrom elliptical cross-section) that ensures rapid compound flux, and solved crystallographic structure. Two areas of application were targeted as proof of principle: (A) selective product recovery in nanocompartments and (B) enzymatic conversion in nanocompartments. Selective recovery of negatively charged compounds has been achieved on the example of sulforhodamine B by using positively charged polylysine molecules as trap inside the nanocompartment. Conversion in nanocompartments has been achieved by 3,3 ',5,5 '-tetrainethylbenzidine oxidation employing horseradish peroxidase (HRP).
Faculties and Departments:05 Faculty of Science > Departement Chemie
05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)
UniBasel Contributors:Meier, Wolfgang P.
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:18 Mar 2020 14:23
Deposited On:18 Mar 2020 14:22

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