Nallani, Madhavan and Benito, Samantha Mariela and Onaca-Fischer, Ozana and Graff, Alexandra and Lindemann, Marcus and Winterhalter, Mathias and Meier, Wolfgang and Schwaneberg, Ulrich. (2006) A Nanocompartment system (Synthosome) Designed for Biotechnical Applications. Journal of Biotechnology, 123 (1). pp. 50-59.
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Official URL: https://edoc.unibas.ch/72416/
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Abstract
A nanocompartment system based on two deletion mutants of the large channel protein FhuA (FhuA Delta 1-129; FhuA Delta 1-160) and an ABA triblock copolymer (PMOXA-PDMS-PMOXA) has been developed for putative biotechnological applications. FhuA is ideally suited for applications in biotechnology due to its monomeric structure, large pore diameter (39-46 angstrom elliptical cross-section) that ensures rapid compound flux, and solved crystallographic structure. Two areas of application were targeted as proof of principle: (A) selective product recovery in nanocompartments and (B) enzymatic conversion in nanocompartments. Selective recovery of negatively charged compounds has been achieved on the example of sulforhodamine B by using positively charged polylysine molecules as trap inside the nanocompartment. Conversion in nanocompartments has been achieved by 3,3 ',5,5 '-tetrainethylbenzidine oxidation employing horseradish peroxidase (HRP).
| Faculties and Departments: | 05 Faculty of Science > Departement Chemie 05 Faculty of Science > Departement Chemie > Former Organization Units Chemistry > Makromolekulare Chemie (Meier) |
|---|---|
| UniBasel Contributors: | Meier, Wolfgang P. |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Elsevier |
| ISSN: | 0168-1656 |
| e-ISSN: | 1873-4863 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Identification Number: |
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| Last Modified: | 18 Mar 2020 14:23 |
| Deposited On: | 18 Mar 2020 14:22 |
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