Virus-assisted loading of polymer nanocontainer

Graff, Alexandra and Sauer, Marc and Van Gelder, Patrick and Meier, Wolfgang. (2002) Virus-assisted loading of polymer nanocontainer. Proceedings of the National Academy of Sciences, 99 (8). pp. 5064-5068.

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Official URL: https://edoc.unibas.ch/72399/

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We present a DNA-containing polymeric nanocontainer using the self-assembled superstructure of amphiphilic block copolymers in aqueous solutions. To demonstrate that DNA translocation is possible across a completely synthetic block copolymer membrane, we have used a phage transfection strategy as a DNA-transfer model system. For this purpose the bacterial channel forming protein LamB was reconstituted in ABA-triblock copolymer vesicles. The outer membrane protein LamB is a specific transporter for maltodextrins but also serves as a receptor for λ phage to trigger the ejection of λ phage DNA. We demonstrate that the functionality of the LamB protein is fully preserved despite the artificial surrounding. This leads to a type of polymeric vehicle for DNA that could be useful for gene therapy. PDMS,poly(dimethylsiloxane);PMOXA,poly(2-methyloxazoline);octyl-POE,octyl-polyoxyethylene
Faculties and Departments:05 Faculty of Science > Departement Chemie
05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)
UniBasel Contributors:Meier, Wolfgang P.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:National Academy of Sciences
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:19 Mar 2020 12:11
Deposited On:19 Mar 2020 12:11

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