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A new water soluble copper N-heterocyclic carbene complex delivers mild O6G-selective RNA alkylation

Rasale, Dnyaneshwar and Patil, Kiran and Sauter, Basilius and Geigle, Stefanie and Zhanybekova, Saule and Gillingham, Dennis. (2018) A new water soluble copper N-heterocyclic carbene complex delivers mild O6G-selective RNA alkylation. Chemical Communications , 54 (66). pp. 9174-9177.

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Official URL: https://edoc.unibas.ch/72357/

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Abstract

We show here that copper carbenes generated from diazo acetamides alkylate single RNAs, mRNAs, or pools of total transcriptome RNA, delivering exclusively alkylation at the O 6 position in guanine (O 6 G). Although the reaction is effective with free copper some RNA fragmentation occurs, a problem we resolve by developing a novel water-stable copper N-heterocyclic carbene complex. Carboxymethyl adducts at O 6 G are known mutagenic lesions in DNA but their relevance in RNA biochemistry is unknown. As a case-in-point we re-examine an old controversy regarding whether O 6 G damage in RNA is susceptible to direct RNA repair.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Organische Chemie (Gillingham)
UniBasel Contributors:Sauter, Basilius and Rasale, Dnyaneshwar and Patil, Kiran and Geigle, Stefanie and Zhanybekova, Saule and Gillingham, Dennis
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Royal Society of Chemistry
ISSN:1359-7345
e-ISSN:1364-548X
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:06 Nov 2019 15:02
Deposited On:06 Nov 2019 15:02

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