Comparative proteomics of stenotopic caddisfly Crunoecia irrorata identifies acclimation strategies to warming

Ebner, Joshua Niklas and Ritz, Danilo and von Fumetti, Stefanie. (2019) Comparative proteomics of stenotopic caddisfly Crunoecia irrorata identifies acclimation strategies to warming. Molecular Ecology, 28 (19). pp. 4453-4469.

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Species' ecological preferences are often deduced from habitat characteristics thought to represent more or less optimal conditions for physiological functioning. Evolution has led to stenotopic and eurytopic species, the former having decreased niche breadths and lower tolerances to environmental variability. Species inhabiting freshwater springs are often described as being stenotopic specialists, adapted to the stable thermal conditions found in these habitats. Whether due to past local adaptation these species have evolved or have lost intra‐generational adaptive mechanisms to cope with increasing thermal variability has, to our knowledge, never been investigated. By studying how the proteome of a stenotopic species changes as a result of increasing temperatures we investigate if the absence or attenuation of molecular mechanisms is indicative of local adaptation to freshwater springs. An understanding of compensatory mechanisms is especially relevant as spring‐specialists will experience thermal conditions beyond their physiological limits due to climate change. In this study, the stenotopic species  Crunoecia irrorata (Trichoptera: Lepidostomatidae, Curtis 1834) was acclimated to 10, 15 and 20 °C for 168 h. We constructed a homology‐based database, and via liquid chromatography‐tandem mass spectrometry (LC‐MS/MS)‐based shotgun proteomics identified 1358 proteins. Differentially abundant proteins and protein norms of reaction revealed candidate proteins and molecular mechanisms facilitating compensatory responses such as trehalose metabolism, tracheal system alteration, and heat shock protein regulation. A species‐specific understanding of compensatory physiologies challenges the characterization of species as having narrow tolerances to environmental variability if that characterization is based on occurrences and habitat characteristics alone.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Geowissenschaften > Geoökologie (Heiri)
UniBasel Contributors:Ebner, Joshua Niklas and von Fumetti, Stefanie and Ritz, Danilo
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:18 Aug 2020 12:32
Deposited On:18 Aug 2020 12:32

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