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A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum

Schindler, Tobias and Deal, Anna C. and Fink, Martina and Guirou, Etienne and Moser, Kara A. and Mwakasungula, Solomon M. and Mihayo, Michael G. and Jongo, Said A. and Chaki, Prosper P. and Abdulla, Salim and Valverde, Paulo C. Manrique and Torres, Katherine and Bijeri, Jose R. and Silva, Joana C. and Hoffman, Stephen L. and Gamboa, Dionicia and Tanner, Marcel and Daubenberger, Claudia. (2019) A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum. Scientific reports, 9. p. 13107.

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Abstract

The rapid and accurate diagnosis of Plasmodium falciparum malaria infection is an essential factor in malaria control. Currently, malaria diagnosis in the field depends heavily on using rapid diagnostic tests (RDTs) many of which detect circulating parasite-derived histidine-rich protein 2 antigen (PfHRP2) in capillary blood. P. falciparum strains lacking PfHRP2, due to pfhrp2 gene deletions, are an emerging threat to malaria control programs. The novel assay described here, named qHRP2/3-del, is well suited for high-throughput screening of P. falciparum isolates to identify these gene deletions. The qHRP2/3-del assay identified pfhrp2 and pfhrp3 deletion status correctly in 93.4% of samples with parasitemia levels higher than 5 parasites/µL when compared to nested PCR. The qHRP2/3-del assay can correctly identify pfhrp2 and pfhrp3 gene deletions in multiple strain co-infections, particularly prevalent in Sub-Saharan countries. Deployment of this qHRP2/3-del assay will provide rapid insight into the prevalence and potential spread of P. falciparum isolates that escape surveillance by RDTs.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Clinical Immunology (Daubenberger)
UniBasel Contributors:Schindler, Tobias and Deal, Anna and Guirou, Etienne and Tanner, Marcel and Daubenberger, Claudia
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Springer Nature
ISSN:0169-5487
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:18 Sep 2019 07:33
Deposited On:18 Sep 2019 07:33

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