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Safety and tolerance of lymph node biopsies from chronic HIV-1 volunteers in rural Tanzania

Mkindi, Catherine Gerald and Marandu, Elias Antony and Masawa, Ngisi and Bani, Farida and Nyuri, Amina and Byakuzana, Theonestina and Klimkait, Thomas and Ding, Song and Pantaleo, Giuseppe and Battegay, Manuel and Orlova-Fink, Nina and Weisser-Rohacek, Maja and Daubenberger, Claudia. (2019) Safety and tolerance of lymph node biopsies from chronic HIV-1 volunteers in rural Tanzania. BMC Research Notes, 12. p. 561.

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Abstract

HIV-1 rapidly establishes a persistent infection that can be contained under life-long antiretroviral therapy (ART) but not cured. One major viral reservoir is the peripheral lymph node (LN) follicles. Studying the impact of novel HIV-1 treatment and vaccination approaches on cells residing in germinal centers is essential for rapid progress towards HIV-1 prevention and cure.; We enrolled 9 asymptomatic adult volunteers with a newly diagnosed HIV-1 infection and CD4 T cell counts ≥ 350/ml. The patients underwent venous blood collection and inguinal lymph node excision surgery in parallel. Mononuclear cells were extracted from blood and tissues simultaneously. Participants were followed up regularly for 2 weeks until complete healing of the surgical wounds. All participants completed the lymph node excision surgery without clinical complications. Among the 9 volunteers, one elite controller was identified. The number of mononuclear cells recovered from lymph nodes ranged from 68 to 206 million and correlated positively with lymph node size. This is the first study to show that lymph node biopsy is a safe procedure and can be undertaken with local experts in rural settings. It provides a foundation for detailed immune response investigations during future clinical trials.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Clinical Immunology (Daubenberger)
UniBasel Contributors:Mkindi, Catherine and Orlova-Fink, Nina and Weisser, Maja and Daubenberger, Claudia
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:BioMed Central
e-ISSN:1756-0500
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:18 Sep 2019 07:29
Deposited On:18 Sep 2019 07:29

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