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PDMS-PMOXA-Nanoparticles Featuring a Cathepsin B-Triggered Release Mechanism

Ehrsam, Daniel and Porta, Fabiola and Hussner, Janine and Seibert, Isabell and Meyer Zu Schwabedissen, Henriette E.. (2019) PDMS-PMOXA-Nanoparticles Featuring a Cathepsin B-Triggered Release Mechanism. Materials, 12 (17). p. 2836.

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Official URL: https://edoc.unibas.ch/71915/

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Abstract

It was our intention to develop cathepsin B-sensitive nanoparticles for tumor-site-directed release. These nanoparticles should be able to release their payload as close to the tumor site with a decrease of off-target effects in mind. Cathepsin B, a lysosomal cysteine protease, is associated with premalignant lesions and invasive stages of cancer. Previous studies have shown cathepsin B in lysosomes and in the extracellular matrix. Therefore, this enzyme qualifies as a trigger for such an approach.; Poly(dimethylsiloxane)-b-poly(methyloxazoline) (PDMS-PMOXA) nanoparticles loaded with paclitaxel were formed by a thin-film technique and standard coupling reactions were used for surface modifications. Despite the controlled release mechanism, the physical properties of the herein created nanoparticles were described. To characterize potential in vitro model systems, quantitative polymerase chain reaction and common bioanalytical methods were employed.; Stable paclitaxel-loaded nanoparticles with cathepsin B digestible peptide were formed and tested on the ovarian cancer cell line OVCAR-3. These nanoparticles exerted a pharmacological effect on the tumor cells suggesting a release of the payload.
Faculties and Departments:05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Pharmazie > Biopharmazie (Meyer zu Schwabedissen)
UniBasel Contributors:Meyer zu Schwabedissen, Henriette and Hussner, Janine and Seibert, Isabell and Porta, Fabiola and Ehrsam, Daniel
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:MDPI
e-ISSN:1996-1944
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:18 Aug 2020 12:17
Deposited On:18 Aug 2020 12:17

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