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Analysis of protein modifications in biopharmaca by capillary electrophoresis and high-performance liquid chromatography : monitoring deamidation, charge variant distribution, and PEGylation

Ferenc, Alena. Analysis of protein modifications in biopharmaca by capillary electrophoresis and high-performance liquid chromatography : monitoring deamidation, charge variant distribution, and PEGylation. 2017, Doctoral Thesis, University of Basel, Faculty of Science.

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Official URL: http://edoc.unibas.ch/diss/DissB_13252

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Abstract

This thesis focuses on the analysis of protein modifications and protein isoforms in biopharmaceuticals. It evaluates the potential of HPLC-UV, HPLC-ESI-MS, CE-UV and CE-ESI-MS methods, when optimized and used together with specific enzymatic treatments, for characterizing protein modifications like deamidation, modifications leading to a shift in the charge pattern, and on PEGylation.
Advisors:Hauser, Peter C. and Schwarz, Maria Anna and Rizzi, Andreas
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser)
UniBasel Contributors:Hauser, Peter C.
Item Type:Thesis
Thesis Subtype:Doctoral Thesis
Thesis no:13252
Thesis status:Complete
Bibsysno:Link to catalogue
Number of Pages:1 Online-Ressource (162 Seiten)
Language:English
Identification Number:
Last Modified:06 Sep 2019 04:30
Deposited On:05 Sep 2019 08:34

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