edoc

Computational evaluation of oxygen and shear stress distributions in 3D perfusion culture systems : Macro-scale and micro-structured models

Cioffi, M. and Küffer, J. and Ströbel, S. and Dubini, G. and Martin, I. and Wendt, D.. (2008) Computational evaluation of oxygen and shear stress distributions in 3D perfusion culture systems : Macro-scale and micro-structured models. Journal Of Biomechanics, 41 (14). pp. 2918-2925.

[img] PDF
Restricted to Repository staff only

1213Kb

Official URL: http://edoc.unibas.ch/dok/A5248795

Downloads: Statistics Overview

Abstract

We present a combined macro-scale/micro-scale computational approach to quantify oxygen transport and flow-mediated shear stress to human chondrocytes cultured in three-dimensional scaffolds in a perfusion bioreactor system. A macro-scale model was developed to assess the influence of the bioreactor design and to identify the proper boundary conditions for the micro-scale model. The microscale model based on a micro-computed tomography (pCT) reconstruction of a poly(ethylene glycol terephthalate)/poly(butylene terephthalate) (PEGT/PBT) foam scaffold, was developed to assess the influence of the scaffold micro-architecture on local shear stress and oxygen levels within the scaffold pores, Experiments were performed to derive specific oxygen consumption rates for constructs perfused under flow rates of 0.3 and 0.03 ml min(-1). While macro-scale and micro-scale models predicted similar average oxygen levels at different depths within the scaffold, mu CT models revealed small local oxygen variations within the scaffold micro-architecture. The combined macro-scale/micro-scale approach indicated that 0.3 ml min(-1), which subjected 95 less than 6.3 mPa shear, would maintain the oxygen supply throughout the scaffold above anoxic levels (< 1 supplied with 8-2 macro-scale model predicted 6 0.5-1 periphery of the scaffold. Together with local variations predicted by the micro-scale model, the simulations underline that in the current model system, reducing the flow below 0.03 ml min(-1) would likely have dire consequences on cell viability to pronounced regions within the engineered construct. The presented approach provides a sensitive tool to aid efficient bioreactor optimization and scaffold design. (c) 2008 Elsevier Ltd. All rights reserved.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
UniBasel Contributors:Martin, Ivan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier Science
ISSN:0021-9290
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Related URLs:
Identification Number:
Last Modified:28 Sep 2017 09:14
Deposited On:22 Mar 2012 13:37

Repository Staff Only: item control page